Fig. 2.

TRPM7 is primarily responsible for spontaneous and store depletion-induced Ca²⁺ influx in oocytes and eggs. (A) Spontaneous Ca²⁺ influx into control and Trpm7 cKO GV oocytes. (Left) Representative traces; (Right) mean area under the curve ± SEM. n = 6 biological replicates, a total of 60 control and 59 Trpm7 cKO oocytes were imaged. *P < 0.05, Mann–Whitney U test. (B and C) Measurements of ER Ca²⁺ stores and Ca²⁺ influx after thapsigargin (Tg)-induced Ca²⁺ store depletion in control and Trpm7 cKO GV oocytes (B) or MII eggs (C). (Left) Representative traces; (Right) mean area under the curve ± SEM following Tg addition and following Ca²⁺ addition. Six biological replicates were performed for GV oocytes, and three for MII eggs, and the total number of cells in each group was: control GV oocytes, 46; Trpm7 cKO GV oocytes, 45; control MII eggs, 33; and Trpm7 cKO MII eggs, 35. *P < 0.05, Mann–Whitney U test (B), t test with Welch’s correction (C).