Fig. 5.

Combined loss of TRPM7 and Ca_V3.2 reduces Ca²⁺ oscillation frequency following ICSI, reduces litter sizes, and alters offspring growth variance. (A and B) Ratiometric Ca²⁺ imaging of 10 wild-type and 23 dKO eggs was performed immediately following ICSI. (A) Representative traces. (B) Number of Ca²⁺ oscillations during the 120-min period following ICSI, mean ± SEM, *P < 0.05, t test with Welch’s correction. (C and D) Preimplantation development of pronuclear-stage embryos derived from wild-type sperm and eggs of the indicated genotypes following in vivo fertilization and zygote collection (C) or in vitro fertilization (D); n = 6–9 females per group. 2C, two-cell stage; 4–8C, 4- to 8-cell stage; blast, blastocyst; mor, morula. (E–G) Breeding trial of wild-type males bred for 6 mo to females carrying either Ca_V3.2 KO or dKO oocytes; seven breeding pairs per group. (E) Average number of live pups per litter, mean ± SEM, *P < 0.05, Mann–Whitney U test. (F) Weight of all male and female offspring over time, mean ± SEM, mixed-model ANOVA. ns, not significant. N = 22 female pups, 20 male pups from Ca_V3.2 KO dams; 15 female pups, 16 male pups from dKO dams. (G) SD of offspring weight over time. Different letters indicate statistically different SDs between groups (mixed-model ANOVA).