Table 2.
Gene expression type | Microarray | RNA-Seq | TempO-Seq |
---|---|---|---|
Platform | Affymetrix whole genome GeneChip Rat Genome 230 2.0 | Illumina HiScan & HiSeq 2000 | BioSpyder S1500+ Beta |
Technology | In situ oligonucleotide array | Next generation nucleotide chain termination sequencing by synthesis | Templated oligonucleotide detection |
Gene content/gene model | ~31,000 gene probe sets | ~38,100 AceView transcripts | ~2,200 Refseq genes |
Normalization | RMA | Magic normalized index | TPM |
Transformation | Log2 | Log2 | Log2 |