Table 2. Cross-Reactivity Data of R12.45 Trunc. Aptamer Candidate^a.

target	normalized average fluorescence	standard deviation	p-value	selectivity ratio
atrazine	0.0716	0.0074
simazine	0.0389	0.0095	0.031	1.84
propazine	0.0218	0.022	0.047	3.23
terbuthylazine	0.0213	0.023	0.048	3.32
cyanazine	–0.02178	0.035	0.033	Neg.b

^aFor each negative target (1 μM), 1 × standard deviation is presented with normalized average fluorescence. Each set of experiments was performed in duplicate. A one-tailed student’s t-test was performed between atrazine and negative targets to determine the statistical significance (p < 0.05). The selectivity ratio represents binding to atrazine is higher than the negative targets.

^bNeg. denotes minimal binding of R12.45 Trunc. to cyanazine, and therefore a very large selectivity ratio.