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. 2018 Oct 3;19(1):foy107. doi: 10.1093/femsyr/foy107

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© FEMS 2018.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com.

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Figure 2. — Physiological characterization of the CRISPR-Hxt⁰ strain IMX1812 and its ancestors. (A) Growth in aerobic shake-flasks with chemically defined medium using maltose as sole carbon source. CEN.PK2-1C: control strain without SpCas9, IMX672: control strain constitutively expressing SpCas9 from its genomic DNA, IMX1812: CRISPR-Hxt⁰ strain and EBY.VW4000: Hxt⁰ strain from Wieczorke et al. (1999). All strains are quadruple auxotrophs and were supplied with uracil, leucine, histidine and tryptophan. Data represent the average and mean deviation of biological duplicates for each strain and growth condition. The asterisk indicates a P value below 0.05 (Student t-test) as compared to the control strain CEN.PK2-1C. (B) Growth on chemically defined medium with various carbon sources. IMX1521 and IMX1541 were obtained after the first and second transformation round, respectively. The pictures were taken after 2 days of incubation at 30°C.