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. 2018 Oct 29;9:2377. doi: 10.3389/fimmu.2018.02377

Figure 3.

Figure 3

Immune cell infiltrate is increased in the lungs of coinfected mice. Innate immune cell populations were quantified in the lungs of mice on days 1 and 3 post-K. oxytoca infection. We identified neutrophils as Ly6G+F480− cells and separated the F480+ population according to expression of CD11c with alveolar macrophages and repopulating macrophages identified as Ly6G−F480+CD11c+ cells. The CD11c− population was further separated by expression of Ly6C and MHC II with inflammatory monocytes identified as Ly6G−F480+CD11c−Ly6C+MHC II− cells and infiltrating macrophages identified as Ly6G−F480+CD11c−Ly6C+MHC II+ cells (A–C). The total number of neutrophils (D), inflammatory monocytes (E), and infiltrating macrophages (F) from days 1 and 3 were calculated according to their percentages of the total population and the total number of cells collected in each lung. @ denotes P ≤ 0.05 between coinfected and uninfected groups. # denotes P ≤ 0.05 between coinfected and influenza groups. * denotes P ≤ 0.05 between coinfected and K. oxytoca groups. Data were analyzed with ANOVA followed by Tukey's multiple comparison tests. Error bars represent SEM. Data are combined from at least four independent experiments with at least four mice per group.