Figure 3.

C9orf72 negatively regulates CARM1 protein level. (A,B) After glucose starvation for 0, 6, and 16 h, wild-type and C9KO MEFs were lysed, and CARM1 expression was determined by immunoblotting. n = 3. (C) Flag-tagged human C9orf72 was expressed in HEK293 cells, which were grown with or without glucose starvation for 6 h. The cells were then analyzed for CARM1 expression by immunoblotting. (D) The rescue of C9orf72 levels by stably expressing human C9orf72 in C9KO MEFs reversed the increase in CARM1 protein level under 6-h glucose starvation back to the wild-type level. (E,F) Cerebral cortex lysates from 2-mo-old wild-type and C9KO mice with or without 24-h fasting conditions were analyzed for CARM levels by immunoblotting. n = 3. (GS) Glucose starvation. Data are presented as mean ± SEM. (*) P < 0.05; (**) P < 0.01.