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. 2018 Nov 1;32(21-22):1398–1419. doi: 10.1101/gad.314377.118

Figure 4.

Figure 4.

PIN1 and MYC recruit GCN5 to the NPC, and PIN1 facilitates mitogen-induced MYC–MAX–GCN5 association with the NPC. (A) PLA of GCN5 association with TPR and pS62MYC in HeLa cells. (B) PLA of TPR–GCN5 association in HeLa cells transfected with control nontargeting (NT) and MYC and PIN1 targeted siRNAs as indicated. (C) Quantification of B showing PLA signals per cell from 50 cells per condition. The whiskers represent 5%–95% intervals, and the box represents median and 25%–75% intervals. Data are representative of three independent experiments. (D) Western blots of the indicated proteins coimmunoprecipitated with TPR using a TPR-specific antibody (a-TPR) in HeLa cells transfected with nontargeting and PIN1 targeted siRNAs as indicated. IgG control immunoprecipitation and 0.5% volume input are also shown. (E, from top to bottom) PLA of MYC association with TPR, GCN5, MAX, and PIN1 in wild-type and PIN1 knockout primary MEFs at the indicated time points after serum stimulation. Serum starvation and stimulation were conducted as in Supplemental Figure S4. (F) Quantification of E showing the average number of PLA signals per cell from 50 cells per condition. The error bars indicate standard errors of three independent experiments. P-values are shown for relevant significant comparisons. (*) P < 0.05; (**) P < 0.01; (***) P < 0.001.