Table 2.
Quantitative proteomics by the Tandem Mass Tag (TMT) method
| Functional grouping | Protein | Fold change (miR-99a-5p/NC) |
|---|---|---|
| Regulation of catalytic activity | Superoxide dismutase | 2.32 |
| Serine/threonine-protein phosphatase | 2.23 | |
| Antigen processing and presentation of peptide antigen via MHC class I | Antigen peptide transporter 1 | 2.01 |
| GTP-binding protein SAR1b | 1.70 | |
| Mitochondrion organization | Stomatin-like protein 2 | 1.87 |
| Single-stranded DNA-binding protein | 1.70 | |
| Cell-cell adhesion | 60S ribosomal protein L29 | 1.85 |
| 60S ribosomal protein L15 | 1.72 | |
| Acyl-protein thioesterase 2 | 1.62 | |
| ER to Golgi vesicle-mediated transport | Dynactin subunit 1 | 1.72 |
| Protein transport protein Sec23B | 1.66 | |
| rRNA processing | 40S ribosomal protein S27 | 1.64 |
| Translation | 40S ribosomal protein S26 | 1.60 |
| Translational initiation | ||
| Negative regulation of blood coagulation | PAI-1 | 1.62 |
| Extracellular matrix organization | Vitronectin | 1.53 |
| Fibronectin | 1.52 | |
| Other | Dipeptidyl peptidase 4 | 2.03 |
| Thioredoxin-like protein 1 | 1.88 | |
| THO complex subunit 4 | 1.79 | |
| Synaptosomal-associated protein 23 | 1.66 | |
| Tetraspanin | 1.64 | |
| Cofilin-2 | 1.63 |
Fold-changes in expression levels of each protein between HPMCs transfected with miR-99a-5p and those transfected with negative control miRNA are listed. Proteins were categorized by gene ontology (GO) based on biological process (BP) using DAVID 6.8 Functional Annotation Bioinformatics Analysis