Figure 9.

NOESY cross peaks observed for Leu73 and Ala69 in H46L/Q47L mesoGlbN. Portions of the ¹³CH_- selective NOESY data displaying the CH_- groups of Leu73 and Ala69 are shown on the left. Cross peaks to Phe50 H^β3, Gln43 H^α, Met66 H^α, Leu47 H^α, Phe50 H^δ1/δ2 and His70 H^α are labeled. Unlabeled peaks correspond to intraresidue NOEs. The relevant residues are shown on the right using the crystal structure of cyanomet GlbN (PDB ID: 1S69).¹⁴ Leu73 and Ala69 are colored purple. The Gln47Leu replacement was generated with Chimera.⁶¹ In this structure the distance between Leu7- and Leu47 is greater than 15 Å. The observed NOEs require the relative repositioning of the E and F helices and the removal of the intervening heme.