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. Author manuscript; available in PMC: 2019 Oct 9.
Published in final edited form as: Biochemistry. 2018 Sep 28;57(40):5785–5796. doi: 10.1021/acs.biochem.8b00752

Table 1.

Selected 1H chemical shifts and T1 values in H46L/Q47L GlbN and H46L/Q47L GlbN mesoGlbNa

MesoH46L/Q47L H46L/Q47L
Residue Nucleus δ (ppm) T1 (ms) δ (ppm) T1 (ms)
His70 NH 10.39 10.35
Hα 7.97 7.80
Hβ3 12.32 45 12.55 35
Hβ2 8.02 8.10
N 120.1 119.8
Cα 82.9 84.8
Cβ 22.5 22.2
Hisll7 NH 11.49 11.23
Hα 7.78 60 7.49
Hβ3 11.70 50 11.56 35
Hβ2 8.18 8.39
N 119.6 119.2
Cα 73.7 74.2
Cβ 20.9 N.D.
a

25 °C, 5% 2H2O, pH 7.1. T1 values have a standard error of the fit lower than 5% (Figure S1).