Table 2.

Primers used to amplify halI and halR genes by PCR.

Primer	Sequence (5'-3')	Application
halI-F	AACTGATTACACCAATGCAGT	Amplification of halI
halI-R	GGAATGCTTGAACTATTTGATG	Amplification of halI
halI-bam	ATTGGATCCTACACCAATGCAGTCTTAATT	Amplification of halI gene and preparation for cloning in pQE-30Xa
halI-sac	ATTGAGCTCATGCTTGAACTATTTGATGTC	Amplification of halI gene and preparation for cloning in pQE-30Xa
halR-F	CTT CAG GGA TGC CAT ATG TTT	Amplification of halR
halR-R	ACT GCA TTG GTG TAA TCA GTT	Amplification of halR

The introduced restriction sites for BamHI and SacI are underlined.