Table 5.
Summary of identification by high-performance liquid chromatography positive electrospray ionization (ESI+-) MS of AHLs produced by H. alvei 059, 068, and 071, Enterobacter sp. 067, and A. hydrophila 099.
| Standard | [M+H]+1 | Retention time of AHL molecules [min] | ||||||
|---|---|---|---|---|---|---|---|---|
| Standard mix Calibration1 | 067 | 068 | 071 | 099 | Standard mix Calibration | 059 | ||
| C4-HSL | 172 | 5.5 | -3 | - | - | 5.8 | 4.29 | - |
| 3-hydroxy-C4-HSL | 188 | Nd2 | - | 4.6 | - | - | Nd | - |
| C5-HSL | 186 | Nd | - | - | - | 7.8 | Nd | - |
| 3-Oxo-C6-HSL | 214 | 6.6 | - | 6.5 | 6.6 | - | 4.57 | 4.55 |
| C6-HSL | 200 | 10.9 | - | 10.9 | 11.0 | 10.9 | 9.69 | 9.45 |
| 3-Oxo-C8-HSL | 242 | 12.5 | - | 12.5 | 12.5 | - | 11.18 | 11.16 |
| C8-HSL | 228 | 15.3 | - | - | 15.4 | - | 14.26 | 14.2 |
| 3- hydroxy-C12-HSL | 300 | Nd | - | 17.2 | 17.2 | - | Nd | - |
| C10-HSL | 256 | 18.6 | - | - | - | - | 17.59 | - |
| 3-Oxo-C12-HSL | 298 | 19.0 | - | - | - | - | Nd | - |
1[M+H]+, mass to charge rate. 2Nd, not determined. 3Nothing found.