Figure 12.

In vitro enzymatic activation of fluorescence of NPs prepared with (A) PLGA-PLL and (B) PLGA-PDL (uncleavable control).

Notes: Inset in B shows data at low fluorescence intensity in more detail. Data plotted are average fluorescence at 780 nm vs time for NPs exposed to trypsin, trypsin + TLCK (trypsin inhibitor), or buffer. The final concentration of trypsin was 6.25 mg/mL and that of TLCK 10 mg/mL. Error bars represent SD between replicates (n=4). *Statistically significant differences in fluorescence at 240 minutes compared to positive control (P<0.05).

Abbreviations: PDL, poly-d-lysine; PLGA, poly(lactic-co-glycolic acid); PLL, poly-l-lysine.