Fig. 1.

(A) Sequences of the investigated β-wrapin variants aligned to ZAβ₃ and their corresponding dissociation constants for Aβ, α-syn, and IAPP reported here and in Mirecka et al. (2014a), Shaykhalishahi et al. (2015), and Mirecka et al. (2014b). The dissociation constant for ZAβ₃ to α-syn was not detected (n.d.). (B, C, and D) Binding of IAPP to ZAβ₃, AS69, and ZAβ₃_A10E analyzed by SPR. Representative sensorgrams were recorded by injection of ZAβ₃ (B), AS69 (C), or ZAβ₃_A10E (D) at the indicated concentrations onto a flow cell with immobilized IAPP for 90 s, followed by washing with buffer for 600 s. Global fitting to a two-state interaction model is shown in black, yielding K_d values of 1.31 μM, 1.23 μM, or 620 nM for ZAβ₃, AS69, or ZAβ₃_A10E, respectively.