Table 1. Quality control and filtering of reads from 454 and Hiseq sequencing.
Sequencing runs | Total Raw Reads | Quality Filter | Contaminants | High-quality reads |
---|---|---|---|---|
454 Reads (root tips) | 1,086,240 | 196,306 | 81,817 | 808,117 (74.4%) |
Root tip Replicate 1 | 31,803,479 | 4,040,700 | 382,197 | 27,380,582 (86.09%) |
Root tip Replicate 2 | 34,133,444 | 3,794,965 | 419,609 | 29,918,870 (87.65%) |
Root tip Replicate 3 | 34,873,477 | 3,632,386 | 454,067 | 30,787,024 (88.28%) |
Mature root replicate 1 | 25,348,308 | 2,801,923 | 233,118 | 22,313,267 (88.03%) |
Mature root replicate 2 | 37,575,294 | 3,886,507 | 379,931 | 33,308,856 (88.65%) |
Mature root replicate 3 | 29,033,618 | 3,166,220 | 293,685 | 25,573,713 (88.08%) |