Table 2. De novo assemblies of the FLX reads using Newbler, TGICL and Mira.

Assembly parameters	95% identity	96% identity	97% identity	98% identity	99% identity	100% identity	TGICL	Mira
Total contigs (>200bp)	23,418	23,497	24,140	24,986	25,548	22,544	78,413	73,084
Avg contig size	749.87	742.22	722.04	696.92	675.54	660.86	641.53	717.46
N50 (bp)	905	893	857	815	787	737	672	762
Large Contigs >500bp	14,720	14,640	14,866	15,122	15,400	14,314	52,763	53,271
% Large Contigs	62.86	62.31	61.58	60.52	60.28	63.49	67.29	72.89
Large contigs N50	1,046	1,033	994	951	912	835	747	828
Largest Contig size (bp)	7,528	6,892	5,977	6,699	5,598	3,787	3,451	5,199
Assembly size (bp)	17,560,564	17,439,938	17,429,939	17,413,219	17,258,803	14,898,493	50,304,196	52,434,582
Reads used in assembly	697,311	691,259	681,502	662,618	624,961	518,627	686,622	506,290
% Assembled reads	86.31	85.56	84.35	82.02	77.35	64.19	84.99	62.67
Singletons	95,262	100,789	109,525	125,932	159,474	234,805	121,295	110,157
% Singletons	11.79	12.48	13.56	15.59	19.74	29.06	15.01	13.63