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Proteins (in 37.5 mM sodium acetate buffer, pH 5, with 6.25% (w/v) sucrose) were denatured and precipitated. Fe2+ in the supernatant was quantified colorimetrically using Ferene S reagent and calibration with (NH4)2Fe(SO4)2. OA, ovalbumin; Tf, holo-transferrin. Shown are means ± SD (N = 6 independent expression experiments).
