Fig 6. Activity of AtNSP3 mutants with substitutions of proposed iron-binding residues.

Purified proteins were incubated with allylglucosinolate (A) or benzylglucosinolate (B) and myrosinase in 50 mM MES buffer, pH 6.0, supplemented with 0.01 mM Fe²⁺ for 40 min. Activity is expressed as the proportion of simple nitrile formed relative to the total amount (nmol) of detected breakdown products. Shown are means ± SD of N = 3 independent expression experiments.