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. 2018 Nov 5;13(11):e0205755. doi: 10.1371/journal.pone.0205755

Fig 8. Impact of Fe2+ and Fe3+ on specifier protein activity of TaTFP.

Fig 8

Purified TaTFP was incubated with allylglucosinolate and myrosinase (Myr) in 50 mM MES buffer, pH 6.0, with or without 0.01 mM Fe2+ (FeII, (NH4)2Fe(SO4)2) or 0.01 mM Fe3+ (FeIII, NH4Fe(SO4)2) for 40 min. Activity is expressed as the proportion of individual products ((A) epithionitrile, (B) organic thiocyanate) formed relative to the total amount (nmol) of detected breakdown products. Shown are means ± SD of N = 5 independent expression experiments. Different letters above bars indicate a significant difference (p<0.05; ANOVA with Tukey's test).