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Validation of the key genes in AD transgenic mice. Total RNAs were isolated from hippocampal tissues of APP/PS-1 transgenic mice and wild type control mice for qRT-PCR analysis of the expression of key genes identified in WGCNA. A-H) Expression of MT1, MT2, NOTCH2, ADD3, RAB31, EHD1, VCAN, and MSX1 in 6-month-old and 14-month-old mice; n = 3 per group. *p < 0.05, **p < 0.01, ***p < 0.001 versus Con. Data are mean±SEM.
