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. Author manuscript; available in PMC: 2018 Nov 5.
Published in final edited form as: Clin Sci (Lond). 2018 Jul 31;132(14):1615–1627. doi: 10.1042/CS20180398

Figure 3.

Figure 3.

Molecular inhibition of ASK1 in human ASM cells. Western blot analysis showing expression of apoptosis signal kinase 1 (ASK1) in human airway smooth muscle cells obtained from healthy donors upon siRNA knockdown. Protein expression was measured at 24h (A), 48h (B) and 72h (C). Anti-mitogenic effect of ASK1 knockdown was assessed by measuring DNA content in cells treated with either 10% FBS (D), 10ng/ml PDGF (E) or 10ng/ml EGF (F). Results are expressed as mean ± SEM. 4–5 patient samples were used in each treatment group. Data were analyzed by one-way ANOVA followed by Bonferroni’s multiple comparison test; *control vs scrambled siRNA; #scrambled siRNA vs ASK1 siRNA; p<0.05 was considered significant.