Skip to main content
. 2018 Nov 5;13(11):e0206459. doi: 10.1371/journal.pone.0206459

Fig 4. Post Acr-1 treatment resulted in augmented proliferation of allogenic T cell while pre Acr-1 treatment hampers T cell response.

Fig 4

CD4 T cells purified by MACS were labelled with CFSE-dye. Later, BMDM primed with 9μg/ml Acr-1 (AcrMΦpre and AcrMΦpost) were co-cultured with allogenic CD4 T cells for 72h. [A, B] The CD4 T cell proliferation was examined by flowcytometry; [B] supernatant was collected for the estimation of IFN-γ; [C] IL-17 by ELISA. AcrMΦpre suppress, in contrast AcrMΦpost promotes the release of IFN-γ and IL-17 when co-cultured with allogenic (Balb/c) naïve T cell. The data signify as pg/ml (mean±SEM) and are representative of 3 independent experiments. Data were analyzed by one-way ANOVA repeated measure *p<0.05, **p<0.01, ***p<0.001, ****p<0.0001.