Fig 5. Acr1 modulates the phosphorylation of STAT molecules.

Macrophages were cultured with Acr-1 (9μg/ml) during (AcrMΦpre) and after differentiation (AcrMΦpost). The cells were treated by Acr-1 (9μg/mL) for 7 days exposure of culture medium supplemented in a pre-and post-strategy. Later, the cytosolic cell lysate was made and Western blotting was completed to demonstrate the expression of [A] pSTAT-1; [B] pSTAT-4. β-Actin was taken as a loading control. The densitometry data represent fold change in expression. The density of untreated cells was considered as 1. LPS is used as positive control. The data are representative of the 2 independent experiments.