Table 3.
Intra and interassay accuracy and precision of MIH produced by acid hydrolysis of an MIC-protein adduct.
Concentration (mg/kg) | Intraassay (within run) | Interassay (between run) | ||||||
---|---|---|---|---|---|---|---|---|
Accuracy (%)a | Precision (%RSD)a | Accuracy (%)b | Precision (%RSD)c | |||||
Day 1 | Day 2 | Day 3 | Day 1 | Day 2 | Day 3 | |||
0.188 | 100±1.8 | 100±3.9 | 100±2.1 | 3.6 | 6.3 | 8.1 | 100±2.6 | 2.3 |
1.88 | 100±7.1 | 100±1.4 | 100±0.9 | 5.3 | 4.9 | 3.1 | 100±3.1 | 2.9 |
8.75 | 100±8.0 | 100±4.1 | 100±5.1 | 3.7 | 2.3 | 3.9 | 100±5.7 | 1.4 |
QC method validation (N = 5).
Mean of three different days of QC method validation (N = 15).
Between run precision was calculated based on Krouwer and Rabinowitz (43) (N = 15).