Figure 6.

H3K4me3 Remodeling along Neuronal Transdifferentiation and Differentiation

(A) PCA across the union of H3K4me3 sites in Kmt2b^−/− and control transdifferentiating MEFs at 5 and 13 days (in the latter after sorting for PSA-NCAM) and in MEFs prior to transdifferentiation.

(B) Quilt of the GO enrichment analysis regarding the cellular components of the genes both differentially H3K4 trimethylated and expressed (FDR < 0.01, fold change [FC] > 0.5) in Kmt2b^−/− with respect to the control.

(C) Correlation of fold change in 13-day Kmt2b^−/− iNs with fold change in Kmt2b^−/− hippocampal neurons. Only genes significantly dysregulated in both datasets are plotted.

(D) Distribution of fold changes in H3K4me3 in in vivo Kmt2b^−/− hippocampal neurons (Kerimoglu et al., 2017) of sites that lose H3K4me3 in transdifferentiating Kmt2b^−/− versus control cells.

(E) Enrichments for biological processes among genes with decereased H3K4me3 in BAM-treated KO cells but not in CaMKII neurons, against the background of all H3K4me3-marked genes.