Table 1. Staining protocol using Hoechst 33342 and Eosin Y.
| Process | Solution | Time (seconds) |
|---|---|---|
| Stain | Eosin Y | 30 |
| Fix | 95% ethanol | Dipping 10 times |
| Fix | 100% ethanol | Dipping 10 times |
| Wash | Distilled water | 5 |
| Stain | Hoechst 33342 | 30 |
| Wash | 100% ethanol | 3 |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.