Table 2. HDR efficiencies of GFP insertion with blunt-ended PCRs as donors.
| Locus | no. of F1 Rollers (rol-6) | |
|---|---|---|
| Screened | GFP+ | |
| wago-4 | 54 | 1 (1.85%) |
| sago-2 (wago-6) | 119 | 1 (0.84%) |
| ppw-1 (wago-7) | 76 | 1 (1.32%) |
All the donors consist of 120 bp long homology arms on both ends.