Table 2. HDR efficiencies of GFP insertion with blunt-ended PCRs as donors.
Locus | no. of F1 Rollers (rol-6) | |
---|---|---|
Screened | GFP+ | |
wago-4 | 54 | 1 (1.85%) |
sago-2 (wago-6) | 119 | 1 (0.84%) |
ppw-1 (wago-7) | 76 | 1 (1.32%) |
All the donors consist of 120 bp long homology arms on both ends.