Figure 3.

Cln3p, but not Whi5p, imposes the proper relationship between T_G1 and T_d. Scatter plots of T_G1 values on the x-axis against T_d values (y-axis). All plots used the natural logarithms (Ln) of the values for wild-type (WT) (A), whi5∆ (B), or cln3∆ (C) cells, sampled from chemostat cultures several times at each dilution rate, as indicated. For WT and whi5∆ cells, regression lines of the Siegel repeated medians are in red and the slope of the linear models are shown (additional statistical parameters are in Table 1). For cln3∆ cells, the red line shown simply connects the average values at each dilution rate. There is no regression line because the relationship between T_d and T_G1 breaks down, especially at longer generation times. Scatter plots of the relationship of cell size and T_d in WT (D) or cells lacking Whip5 (E) or Cln3p (F), with cell size values (x-axis) plotted against T_d (y-axis) from the same cultures described in (A–C). All the strains were in the BY4741 background (see Materials and Methods).