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Intestinal epithelial cells after removal from basement membrane with EDTA/DTT protocol. (a) Intestinal epithelial cells in Subheading 3.1, step 14 with whole crypts intact. Arrows depict crypts while arrowheads indicate portions of villi. Images are ×40 original magnification. (b) Cell solution after dissociation to single cells in Subheading 3.1, step 17. Images are ×100 original magnification.
