Skip to main content
. Author manuscript; available in PMC: 2018 Nov 5.
Published in final edited form as: Methods Mol Biol. 2012;879:89–107. doi: 10.1007/978-1-61779-815-3_6

Fig. 5.

Fig. 5

Cell sorting of IESCs using Sox9 EGFP and CD24. (a) Gating to exclude debris, lymphocytes, and dead cells. (b) Gating to include only single cells. (c) A univariate graph of Sox9 EGFP expression in cells passing through doublet discrimination. Cells within the “Sox9 EGFPLow” gate are IESCs. (d) FACS analysis of intestinal epithelial cells stained with anti-CD24 antibody. Cells within the “CD24Low” gate are enriched for IESCs.