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. Author manuscript; available in PMC: 2018 Nov 5.
Published in final edited form as: Methods Mol Biol. 2012;879:89–107. doi: 10.1007/978-1-61779-815-3_6

Table 1.

Dissociation reagents for intestinal epithelial prep

Reagent Function
Ethylenediaminetetraacetic acid (EDTA) Chelates Ca2+, breaks cadherin-mediated cell–cell junctions (13)
Dithiothreitol (DTT) Mucolytic/breaks disulfide bonds (14)
Dispase Digests cell–cell junction proteins (22)