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. Author manuscript; available in PMC: 2018 Nov 5.
Published in final edited form as: Methods Mol Biol. 2012;879:89–107. doi: 10.1007/978-1-61779-815-3_6

Table 2.

Growth factors and reagents for in vitro intestinal epithelial stem cell assay

Reagent Category Function
Matrigel Extracellular matrix Structural support of IESCs through laminins (23)
R-spondin 1 (Rspo1) Growth factor Stimulates canonical Wnt signaling (24)
Jagged-1 peptide Growth factor Inhibits premature IESCs differentiation (25)
Noggin 1 Growth factor Known to cause crypt expansion in vivo (26)
Epidermal growth factor (EGF) Growth factor Stimulates intestinal growth in vivo (27)
Wnt3a Growth factor Provides mitogenic signals to IESCs (7)
B27 Media supplement Supplements for serum-free media (28)
N2 Media supplement Supplements for serum-free media (29)
HEPES Media supplement Maintains proper pH of IESC growth media
Y-27632 Anoikis inhibitor Inhibits apoptotic response to epithelial dissociation (30)