Figure 7.
Size-exclusion chromatography of 4DH3 at pH 6.5 (MES 20 mM, NaCl 200 mM), both in the presence (black line) and in the absence (blue line) of 50 µM ZnCl2. In the apo state, two single well-resolved peaks are observed, indicative of a momomer-dimer equilibrium. In the presence of zinc, a single poorly defined peak is produced. Multi-peak fitting (green lines and red dashed lines for the sum) led identification of the aggregation state of the three species under the main peak, resulting in apparent molecular weights corresponding to oligomerization state n = 3.4; 2.0; 0.9, respectively.