Table 3.
Recovery as accuracy studies of the proposed HPTLC Method (n = 6).
| Percent (%) of ursolic acid, β-sitosterol and lupeol added to analyte | Theoretical concentration of ursolic acid, β-sitosterol and lupeol (ng/μL) | Ursolic acid |
β-sitosterol |
Lupeol |
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Concentration found (ng/μL) ± SD | % RSD | % Recovery | Concentration found (ng/μL) ± SD | % RSD | % Recovery | Concentration found (ng/μL) ± SD | % RSD | % Recovery | ||
| 0 | 200 | 198.18 ± 2.16 | 1.089 | 99.09 | 197.14 ± 2.18 | 1.105 | 98.57 | 199.03 ± 2.69 | 1.351 | 99.51 |
| 50 | 300 | 298.02 ± 3.57 | 1.197 | 99.34 | 297.8 ± 4.19 | 1.407 | 99.26 | 299.27 ± 4.18 | 1.396 | 99.75 |
| 100 | 400 | 400.52 ± 4.89 | 1.220 | 100.13 | 398.53 ± 5.81 | 1.457 | 99.63 | 396.07 ± 6.09 | 1.537 | 99.01 |
| 150 | 500 | 498.79 ± 6.19 | 1.240 | 99.75 | 492.03 ± 7.57 | 1.538 | 98.40 | 498.58 ± 7.79 | 1.562 | 99.71 |