Table 5.
Robustness of the proposed HPTLC Method (n = 6).
| Optimization condition | Ursolic acid |
β-sitosterol |
Lupeol |
|||
|---|---|---|---|---|---|---|
| SD | %RSD | SD | %RSD | SD | %RSD | |
| Mobile phase composition; (Chloroform: methanol; 97:3) | ||||||
| (96.5: 3.5) | 2.89 | 0.994 | 3.87 | 1.296 | 5.11 | 1.717 |
| (97: 3) | 2.99 | 1.022 | 3.92 | 1.315 | 5.19 | 1.739 |
| (97.5: 2.5) | 3.02 | 1.029 | 3.99 | 1.336 | 5.27 | 1.771 |
| Mobile phase volume (for saturation) | ||||||
| (18 mL) | 2.89 | 0.997 | 3.76 | 1.265 | 5.26 | 1.746 |
| (20 mL) | 2.87 | 0.978 | 3.79 | 1.274 | 5.29 | 1.756 |
| (22 mL) | 2.91 | 0.988 | 3.81 | 1.280 | 5.31 | 1.763 |
| Duration of saturation | ||||||
| (10 min) | 2.82 | 0.955 | 3.82 | 1.281 | 5.07 | 1.683 |
| (20 min) | 2.86 | 0.965 | 3.84 | 1.290 | 5.03 | 1.670 |
| (30 min) | 2.78 | 0.945 | 3.88 | 1.304 | 5.09 | 1.690 |