Table 6.
HPTLC analysis of ursolic acid, β-Sitosterol and lupeol in different fractions of H. calyphyllus, H. deflersii and H. micranthus.
| S. No. | Samples | Ursolic acid content (µg/mg of dried weight of extract) | β-sitosterol content (µg/mg of dried weight of extract) | Lupeol content (µg/mg of dried weight of extract) |
|---|---|---|---|---|
| 1 | H. deflersii petroleum ether fraction (HdP) | 5.50 ± 0.17 | 11.85 ± 0.26 | 7.47 ± 0.23 |
| 2 | H. micranthus petroleum ether fraction (HmP) | 4.03 ± 0.11 | 8.57 ± 0.25 | 5.37 ± 0.16 |
| 3 | H. calyphyllus petroleum ether fraction (HcP) | 1.51 ± 0.03 | 5.43 ± 0.19 | 0.42 ± 0.004 |
| 4 | H. micranthus toluene fraction (HmT) | 3.20 ± 0.08 | 0.23 ± 0.003 | 0.81 ± 0.007 |
| 5 | H. deflersii toluene fraction (HdT) | Not detected | Not detected | Not detected |
| 6 | H. calyphyllus toluene fraction (HcT) | Not detected | Not detected | Not detected |
| 7 | H. micranthus chloroform fraction (HmC) | Not detected | 0.062 ± 0.0009 | 0.41 ± 0.006 |
| 8 | H. deflersii chloroform fraction (HdC) | 1.27 ± 0.03 | 0.59 ± 0.01 | 0.74 ± 0.01 |
| 9 | H. calyphyllus chloroform fraction (HcC) | 0.06 ± 0.001 | 0.098 ± 0.001 | 0.06 ± 0.001 |
| 10 | H. micranthus ethyl acetate fraction (HmE) | Not detected | Not detected | Not detected |
| 11 | H. deflersii ethyl acetate fraction (HdE) | 1.19 ± 0.02 | 0.049 ± 0.0005 | 0.11 ± 0.001 |
| 12 | H. calyphyllus ethyl acetate fraction (HcE) | 0.015 ± 0.0001 | Not detected | Not detected |
| 13 | H. micranthus n-butanol fraction (HmB) | Not detected | Not detected | Not detected |
| 14 | H. deflersii n-butanol fraction (HdB) | Not detected | Not detected | Not detected |
| 15 | H. calyphyllus n-butanol fraction (HcB) | Not detected | Not detected | Not detected |