Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 30;9:632. doi: 10.3389/fendo.2018.00632

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Khodai, Nunn, Worth, Feetham, Belle, Piggins and Luckman.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

PACAP neurons inside, but not outside the VMH are GI: analysis of PACAP neurons from Pacap-cre::EYFP mice. (A) Representative coronal brain section from a Pacap-cre::EYFP mouse. The accompanying sketch summarizes the distribution of glucose-responsive and non-responsive neurons (PACAP and non-.PACAP) in the ventromedial hypothalamus. (B) Representative image (inset higher magnification) of dual-label immunohistochemistry showing co-localization of nNOS labeling with PACAP^VMH. (C) Whole-cell patch-clamp recordings showing the response to a change in glucose (2.5 mM to 1 mM) on PACAP neurons inside (i) and outside (ii) the VMH. (D) (i) Quantitative (increase in baseline firing frequency; paired t-test, ^*p < 0.05, n = 8) and (ii) qualitative analysis of PACAP^VMH neuron response to low glucose. (E) The neurons respond to low glucose with depolarization in the presence of TTX.