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. 2018 Oct 30;6:500. doi: 10.3389/fchem.2018.00500

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Copyright © 2018 Yoeun, Cho and Han.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chloroplast targeting sequences of AOC in plant species and cellular localization of OsAOC in rice leaves. (A) Comparison of chloroplast targeting sequences (gray) of monocot and dicot AOCs. Accession numbers: OsAOC (AJ493664), HvAOC (AJ308488), TaAOC (KF039887), ZmAOC (AY488136), AtAOC1 (AJ308483), AtAOC2 (AJ308484), AtAOC3 (AJ308485), AtAOC4 (AJ308486), GmAOC1 (HM803106), GmAOC2 (HM803107), GmAOC3 (HM803108), GmAOC4 (HM803109), GmAOC5 (HM803110), GmAOC6 (HM803111). (B) Immunofluorescence confocal microscopy was used to localize OsAOC in rice leaves. The green immunofluorescent signal corresponds to OsAOC. The red fluorescent signal is chlorophyll autofluorescence in the chloroplast. Blue fluorescence is nuclear. DIC indicates a phase contrast image of the microscopic field.