Figure 2.
Expression and localization of different nuclear envelope proteins in myoblasts. (A) Western Blot and quantification of primary control and DM1 myoblasts for emerin, LBR, Sun2 TMEM70, and TMEM38a. DM1 samples are ordered according their diagnosed repeat length from left (small repeat) to right (long repeat). The lanes for DM1-3 and DM1-4 have been rearranged for the LBR, SUN2, TMEM70, and TMEM38a blots to allow a presentation according to repeat length (indicated by black boxes). For original blots see supplemental material. (B) Immunofluorescence staining of primary control and DM1 myoblasts for emerin, LBR, Sun2 TMEM38a TMEM70 and Sun1. Scale bar 10 μm.