Fig. 3.

FTO upregulation mediates m⁶A demethylation in TKI resistant cells. a Dotblotting for mRNA from parental and resistant cells using anti-m⁶A antibody. b, c The eluted mRNA from anti-m⁶A immunoprecipitates in parental and resistant cells was subjected to cDNA synthesis, and gene expression was determined by qPCR. d, e Western blotting of parental and resistant cells. f The parental cells were transfected with FTO expression or control vectors for 48 h. g, h The resistant cells were transfected with FTO shRNA or scramble vectors (g) or treated with 25 µM rhein (h) for 48 h. i K562 nilotinibR cells were treated with 50 µM meclofenamic acid for 48 h. Left, Western blotting for FTO protein expression and dotblotting for m⁶A abundance; right, qPCR for gene expression. Data represent three independent experiments. In f, g and h, the cell lysates were subjected to Western blotting (upper) and the mRNA for dotblotting using anti-m⁶A (lower); sh, shRNA; Vec, vector; Data represent three independent experiments; Data are mean ± SD; *p < 0.05, **p < 0.01. See also Figure S3 and Table S2