Fig. 1.
Postnatal deletion of Mettl3 in hippocampus prolonged the process of long-term memory consolidation. a Characterization of Mettl3 conditional knockout in the CA1 region of 8-week male mouse brains. Scale bars, 100 µm. b Discrimination index of the control (CTRL, Mettl3f/f) and Mettl3 conditional knockout (cKO, Mettl3f/f; CaMKIIα-Cre) mice in novel object recognition test (CTRL, n = 13 mice; cKO, n = 10 mice). c Results of Morris water maze test on five consecutive training days. Group difference was measured by two-way repeated ANOVA, P < 0.001. d Probe tests of the CTRL (blue) and cKO (red) mice during the Morris water maze test. Upper panel, occupancy frequency for the target and nontarget quadrants; lower panel, representative swimming paths of the upper panel. e Freezing behavior before (naïve) and 30 min (short-term) after one-shock fear conditioning. f Freezing behavior during contextual test 24 h after one-shock training (left) or three-shock training (right). In (d), (e), and (f), CTRL, n = 14 mice; cKO, n = 13 mice. Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, N.S., not significant