Fig. 6.

H19 suppresses oestrogen-induced rat pituitary tumour tumourigenesis. a H19 adenovirus has therapeutic effects on rat in situ prolactinomas. When prolactinomas were induced by 17β-estradiol for 6 weeks, tumour-bearing rats were treated with one stereotactic intratumoural injection of 10⁹ pfu adenovirus harbouring the H19 overexpression construct or the control EV. Two weeks later, tumour size was measured by MRI; tumours were then dissected, and tumour volume was calculated. The data are presented as the mean ± SEM (red circles, n = 4; ***p < 0.001), scale bar,1 cm. b Overexpression of H19 suppresses 4E-BP1 phosphorylation in rat in situ prolactinomas. Some of the dissected tumour tissues described in a were homogenized in lysis buffer with a tissue homogenizer; then, the samples were centrifuged, and the supernatant was collected and used for immunoblot analysis with the indicated antibodies. c Representative images of IHC staining show that H19 administration decreases 4E-BP1 phosphorylation, but not S6K1 phosphorylation, and pituitary tumour cell proliferation (Ki-67), scale bar, 500 μm