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. 2018 Nov 5;9:4620. doi: 10.1038/s41467-018-06497-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Cell proliferation and the role of cell division for epithelial folding. a A wing imaginal disc of a 96 h after egg lay (AEL) larva carrying 48 h-old clones of cells marked by the expression of CD8-mCherry (Act5C > Gal4, UAS-CD8-mCherry, red). Adherens junctions are labeled by E-cad-GFP (gray). Scale bar is 10 μm. b Ratio of the average cell number per clone in the pouch and the average cell number per clone in the notum. Mean and s.e.m. are shown. n = 19 wing imaginal discs, 82 clones in the pouch region, and 59 clones in the notum region. c–j Top view (c, d, g, h) and cross-sectional (e, f, i, j) images of time-lapse movies of control (c–f) and Cdk1^E1-24 mutant (g–j) cultured wing imaginal discs expressing E-cad-GFP are shown for the indicated time points after shift to the restrictive temperature. Scale bars are 10 μm