FIGURE 7.

Comparison of Ctip2 labeling with immunofluorescence and chromogenic immunohistochemistry. Subcortical projection neurons of the neocortex (left) can be identified by labeling Ctip2 with AF-488 (green), NeuN with AF-647 (red), and nuclei with DAPI (blue) using immunofluorescence. White box demarcates representative location of Ctip2 and NeuN labeled panels to the right. Immunofluorescence produces clearer and more precise labeling than using the brown chromogen DAB (DAB Peroxidase Substrate Kit, Vector, SK-4100) to label the same rabbit anti-Ctip2 antibody for brightfield microscopy. Fluorophore signal was clearer and easier to discriminate from background labeling and co-labeling with NeuN helped to more clearly identify this neuronal subclass. Scale bar, 100 μm.