Table 2.
Photobleaching experiments.
| Mountant | Exposure duration (min) | Mean gray value | Signal reduction (%) | |
|---|---|---|---|---|
| AF-647 | Mowiol | 0.1 | 6.1 | – |
| 2 | 1.7 | 72.1 | ||
| 15 | 0.0 | 100.0 | ||
| NL-637 | Mowiol | 0.1 | 78.3 | – |
| 2 | 76.0 | 2.9 | ||
| 15 | 58.5 | 25.3 | ||
| Cy2 | Mowiol | 0.1 | 33.8 | – |
| 2 | 2.8 | 91.8 | ||
| 15 | 0.0 | 100.0 | ||
| AF-488 | Mowiol | 0.1 | 61.8 | – |
| 2 | 39.5 | 36.1 | ||
| 15 | 22.0 | 64.3 | ||
| AF-488 | Prolong Gold | 0.1 | 56.7 | – |
| 2 | 44.8 | 20.9 | ||
| 15 | 31.8 | 43.9 | ||
Various fluorophores and mounting media were tested for rates of photobleaching by measuring pixel intensity initially, at 2 min after exposure to replicate a sampling run, and at 15 min to attempt to measure total signal quenching.