Table 1.
Proteins of the seminal plasma differentially expressed in bulls with high (HF) and low (LF) fertility scores. Proteins were identified by DDA (data dependent acquisition) label-free mass spectrometry, Progenesis QI software and UniProt database.
| Accession number | Description | Overexpression in | Peptide count | Unique peptides | Confidence score | Anova (p) |
|---|---|---|---|---|---|---|
| Q05927 | 5′-nucleotidase | LF | 63 | 33 | 173.56 | 0.031 |
| Q50HZ8 | Acetyl-CoA carboxylase, type beta (Fragment) | HF | 1 | 1 | 70.17 | 0.023 |
| Q27984 | Alpha1-antichymotrypsin isoform pHHK12 (Fragment) | LF | 1 | 1 | 47.62 | 0.049 |
| F1MVR5 G3N156 | Anion exchange protein | LF | 1 | 1 | 21.11 | 0.026 |
| Q5EA01 | Beta-1,4-glucuronyltransferase 1 | LF | 4 | 4 | 98,80 | 0.043 |
| H7BWW2 | Beta-hexosaminidase | LF | 37 | 26 | 97.55 | 0.049 |
| F1N619 | Cadherin-1 (Fragment) | LF | 174.00 | 0.012 | ||
| P52193 | Calreticulin | HF | 5 | 5 | 61.38 | 0.049 |
| P06833 | Caltrin | HF | 15 | 13 | 138.93 | 0.020 |
| A6BML7 | Carboxypeptidase | HF | 15 | 15 | 39.83 | 0.008 |
| Q17QK3 | Carboxypeptidase Q | LF | 1 | 1 | 47.79 | 0.047 |
| A5PJF7 | C-C motif chemokine | HF | 3 | 3 | 339.39 | 0.009 |
| F1N2J8 | Chromosome 16 open reading frame 89 | HF | 6 | 6 | 75.18 | 0.032 |
| F1MLR4 | Ciliary neurotrophic factor receptor subunit alpha precursor | LF | 6 | 6 | 121.84 | 0.033 |
| P17697 | Clusterin | LF | 85 | 73 | 1233.10 | 0.027 |
| A0A0F6QNP7 | Complement component 3 | LF | 5 | 5 | 467.05 | 0.040 |
| P81187 | Complement factor B | LF | 6 | 6 | 433.91 | 0.047 |
| F1MC45 | Complement factor H (Fragment) | LF | 61 | 5 | 221.93 | 0.004 |
| P55206 | C-type natriuretic peptide | HF | 46 | 44 | 488.24 | 0.032 |
| A7MBJ5 | Cullin-associated NEDD8-dissociated protein 1 | LF | 22 | 20 | 115.93 | 0.033 |
| P81425 | Dipeptidyl peptidase 4 | LF | 25 | 25 | 303.81 | 0.024 |
| O18738 | Dystroglycan | HF | 2 | 2 | 94.94 | 0.011 |
| E1BJV0 | EH domain containing 4 | LF | 14 | 11 | 142.00 | 0.009 |
| A6QR19 | ENO2 protein | HF | 1 | 1 | 33.46 | 0.042 |
| P79345 | Epididymal secretory protein E1 | LF | 29 | 29 | 123.57 | 0.031 |
| A7E3W2 | Galectin-3-binding protein | LF | 23 | 22 | 313.46 | 0.031 |
| E1BA29 | Guanine nucleotide-binding protein G(q) subunit alhpa | LF | 1 | 1 | 97.95 | 0.008 |
| Q0P565 | HD domain-containing protein 2 | HF | 1 | 1 | 70.52 | 0.005 |
| Q76LV2 | Heat shock protein HSP 90-alpha | HF | 29 | 28 | 189.30 | 0.029 |
| F1MNT3 | Hormone-sensitive lipase | LF | 10 | 8 | 52.37 | 0.004 |
| Q7YS45 | Hyaluronidase (Fragment) | LF | 1 | 1 | 107.36 | 0.029 |
| E1B748 | Hypoxia up-regulated protein 1 precursor | HF | 17 | 17 | 289.84 | 0,001 |
| Q70IB2 | Inactive ribonuclease-like protein 10 | HF | 8 | 8 | 57,69 | 0,029 |
| Q95M12 | Legumain | LF | 8 | 8 | 138.49 | 0,022 |
| Q9MYM4 | Lysosomal alpha-glucosidase | LF | 11 | 11 | 76.30 | 0,036 |
| Q3SZI0 | Mannose-6-phosphate isomerase | HF | 6 | 6 | 84.25 | 0,039 |
| A5D7D5 | MATN2 protein | HF | 4 | 4 | 50.06 | 0.022 |
| E1BDF3 | Matrilin 4 | HF | 20 | 20 | 149.27 | 0.017 |
| P16368 | Metalloproteinase inhibitor 2 | HF | 11 | 11 | 1071.83 | 0.034 |
| Q9N282 | MMP-9 (Fragment) | HF | 1 | 1 | 33.60 | 0.014 |
| Q1LZH9 | N-acetylglucosamine-6-sulfatase | HF | 9 | 9 | 62.80 | 0.040 |
| E1BI74 | NAD(P)(+)−arginine ADP-ribosyltransferase (Fragment) | HF | 14 | 13 | 76.94 | 0.002 |
| Q0IIH5 | Nucleobindin 2 | HF | 26 | 25 | 290.90 | 0.021 |
| Q32KV6 | Nucleotide exchange factor SIL1 | LF | 5 | 5 | 57.08 | 0.029 |
| A7MBI8 | NUDT9 protein | LF | 1 | 1 | 39.35 | 0.049 |
| E1B818 | Olfactomedin-like 2A-like | HF | 6 | 6 | 163.47 | 0.001 |
| Q9BGI2 | Peroxiredoxin-4 | HF | 3 | 3 | 66.36 | 0.021 |
| Q32KN6 | Phosphoglycerate kinase | HF | 35 | 27 | 100.42 | 0.045 |
| Q28017 | Platelet-activating factor acetylhydrolase | HF | 61 | 59 | 1162.20 | 0.026 |
| A1L555 | Prosaposin | HF | 35 | 3 | 120.78 | 0.007 |
| P21856 | Rab GDP dissociation inhibitor alpha | HF | 9 | 4 | 64.47 | 0.033 |
| Q0VCQ9 | Reticulocalbin 2, EF-hand calcium binding domain | HF | 9 | 9 | 65.81 | 0.020 |
| Q0III8 | RNASET2 protein (Fragment) | HF | 6 | 6 | 26.70 | 0.042 |
| A7MB70 | Semaphorin-3C | HF | 6 | 5 | 97.87 | 0.011 |
| P81019 | Seminal plasma protein BSP-30 kDa | HF | 85 | 83 | 2706.25 | 0.005 |
| P00669 | Seminal ribonuclease | HF | 92 | 79 | 1070.13 | 0.018 |
| Q29443 | Serotransferrin | LF | 29 | 1 | 30.41 | 0.029 |
| Q2HJF0 | Serotransferrin-like | HF | 33 | 4 | 208.39 | 0.002 |
| Q862P3 | Similar to cyclophilin B (Fragment) | HF | 1 | 1 | 73.31 | 0.021 |
| F1MJI3 | SLIT-ROBO Rho GTPase activating protein 3 | HF | 2 | 1 | 20.89 | 0.036 |
| Q4R0H2 | Spermadhesin 2 | LF | 80 | 29 | 300.31 | 0.015 |
| F1MHF1 | ST6 beta-galactoside alpha-2,6-sialyltransferase 1 | HF | 13 | 13 | 138.21 | 0.024 |
| P82292 | Spermadhesin Z13 | HF | 53 | 4 | 36.67 | 0.038 |
| A6QQA8 | Sulfhydryl oxidase | HF | 38 | 37 | 405.51 | 0.013 |
| F1MJB6; A0JN68 | Targeting protein for Xklp2 | HF | 1 | 1 | 20.33 | 0.024 |
| Q32L40 | T-complex protein 1 subunit alpha | HF | 1 | 1 | 42.71 | 0.034 |
| Q3ZBH0 | T-complex protein 1 subunit beta | HF | 18 | 18 | 58.09 | 0.021 |
| Q7YRQ8 | Tissue factor pathway inhibitor 2 | LF | 34 | 32 | 378.76 | 0,007 |
| F1MNY2 | Transmembrane protein 2 | HF | 1 | 1 | 22.47 | 0.010 |
| Q3T077 | Tubulin polymerization-promoting protein family member 2 | HF | 5 | 5 | 68.68 | 0,050 |
| G3X861 | Uncharacterized protein (Fragment) | HF | 1 | 1 | 55.44 | 0.050 |
| F1MY12 | Uncharacterized protein (Fragment) | HF | 2 | 2 | 91,12 | 0.010 |
| E1BI55 | Uncharacterized protein | HF | 1 | 1 | 21.69 | 0.039 |
| G5E5W7 | Uncharacterized protein | HF | 1 | 1 | 54.09 | 0.013 |
| Q3MHN5 | Vitamin D-binding protein | LF | 1 | 1 | 70.54 | 0.021 |
| Q32LB7 | V-type proton ATPase subunit E 2 | HF | 1 | 1 | 31.85 | 0.044 |
| P40682 | V-type proton ATPase subunit S1 | HF | 1 | 1 | 60.26 | 0.025 |
| Q3T0Z0 | WAP four-disulfide core domain 2 | HF | 3 | 3 | 98.59 | 0.029 |
| Q3ZCH5 | Zinc-alpha-2-glycoprotein | LF | 2 | 2 | 100.34 | 0.006 |