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. 2018 Oct 30;9:1519. doi: 10.3389/fphys.2018.01519

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Copyright © 2018 Wang, Miao, Ni, Wang, Qian, Yu, Liu, Wang and Yi.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Quantitative real-time PCR analysis of tight junction genes in Schwann cells treated with MMPs. (A) MMP7 mRNA expression in Schwann cells transfected with MMP7 siRNA. (B) MMP9 mRNA expression in Schwann cells transfected with MMP9 siRNA. (C–E) The mRNA expression levels of (C) CLDN1, (D) CLDN10, and (E) CLDN22 in Schwann cells transfected with MMP7 siRNA. (F–H) The mRNA expression levels of (F) CLDN1, (G) CLDN10, and (H) CLDN22 in Schwann cells transfected with MMP9 siRNA. The expression levels of tight junction genes were expressed as relative abundance of target genes normalized to MRPL10 with respect to control. Numerical results were shown as mean (SD). ^∗Statistically different from control (n = 3, paired t-test, P < 0.05).