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. 2018 Oct 30;11:389. doi: 10.3389/fnmol.2018.00389

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Copyright © 2018 Brivio, Sbrini, Peeva, Todiras, Bader, Alenina and Calabrese.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of brain-derived neurotrophic factor (Bdnf) transcripts mRNA levels (A,C,D,E) and mature BDNF protein levels (B) in the crude synaptosomal fraction of prefrontal cortex (PFC) of tryptophan hydroxylase 2 (TPH2^+/+) and TPH2^−/− adult male and female rats. The data, expressed as % of TPH2^+/+ male rats, represent the mean ± standard error mean (SEM) of at least 14 independent determinations for the RNA and six for the western blot. *p < 0.05, **p < 0.01 vs. TPH2^+/+ of the same sex; ^$p < 0.05 vs. TPH2^+/+/male (two-way analysis of variance (ANOVA) with Fisher’s protected least significant difference (PLSD).