Figure 9.

LIC activated human fibroblasts. (A) Immunofluorescent staining of α-smooth muscle actin protein (a specific marker of activated fibroblasts or myofibroblasts) in a primary cell line of cultured human fibroblast treated previously for 48 h with Mock, LIC conditioned-medium, and LIC. Propidium iodide was used for nuclear staining. (B) Representative FACS plots of SMA mean fluorescent intensity (MFI). (C) Representative FACS plots of SMA MFI of a co-culture (Co-) of fibroblast and macrophages infected or not for 48 h with LIC in absence or presence of a non-specific Galectin-3 inhibitor (Gal3i). Bar indicate 50 microns. ^*P < 0.05; ^***P < 0.001.